uMRT Direct RNA-Seq Kit

Get longer reads, detect more genes and isoforms, and experience improved modification signal-to-noise from your direct RNA-Seq. The UltraMarathonRT® (uMRT) Direct RNA-Seq Kit pairs the helicase-driven processivity of uMRT with the Oxford Nanopore Technologies® (SQK-RNA004) workflow.

Why Choose This Kit

• Detect more genes, isoforms, and longer, more contiguous native RNA reads
  High processivity at low temperature preserves RNA integrity and reduces early terminations and read truncation.
• Dramatically improved detection of long transcripts
  Retains long RNAs that regularly degrade at high temperatures (e.g., >50°C).
• Generate Higher cDNA yields from your Sample
  uMRT’s optimized chemistry delivers higher cDNA yields for Direct RNA-Seq.
• Drop-in for ONT workflows
  Fits naturally into the Direct RNA-Seq protocol; no changes to downstream ligation, loading, or basecalling.
• Convenience & Cost-Savings
  One box contains everything you need to pair with ONT’s SQK-RNA004, eliminating the hassle and reducing the cost of ordering multiple reagents from different vendors.
  

Applications

• RNA modification mapping
• Isoform and UTR profiling directly on native RNA
• Viral and host–pathogen transcriptomics
• Long noncoding RNAs and repetitive/high-GC regions

UltraMarathonRT Direct RNA-Seq Kit Components:

• UltraMarathonRT® (20 U/μL)
• 2x RT Reaction Buffer
• dNTP Mix (10 mM each)
• RNA Conditioner
• 5x Ligation Buffer Q
• DNA Ligase (2,000,000 U/mL)
• RNase Inhibitor (40 U/μL)
• Quenching Buffer
• Nuclease-free Water

Reagents Required but Not Supplied:

• Oxford Nanopore Technologies® (ONT) SQK-RNA004 or SQK-RNA004-XL Direct RNA Sequencing kit.
• ONT MinION®/GridION® RNA Flow Cell (FLO-MIN004RA) or PromethION® RNA Flow Cell (FLO-PRO004RA).
• RNA Purification Beads.
• Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851).
• RNA template: 500 ng - 1 μg total cellular RNA or 300 ng of poly (A)+ RNA is recommended for each reaction.

Product Information:

Catalog# R1008S (6 reactions), R1008M (48 reactions)

Storage Conditions: For short duration of storage (< 6 month), UltraMarathonRT can be stored in a -20℃ freezer. -80℃ is recommended for long-term storage. All other kit components are stable in a -20℃ freezer for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.

For Research Use Only.

For the product brochure, click here.

For purchase orders, please contact us at sales@rnaconnect.com.