Achieve a more accurate and comprehensive view of the transcriptome—at the same cost as leading kits. Click the cards below to explore how the cDNA Synthesis & Amplification Kit, powered by uMRT, delivers deeper insights from RNA samples.
Uncover the RNAs that retroviral reverse transcriptases miss. Switch to the UltraMarathonRT-powered cDNA Synthesis & Amplification Kit
RNA-seq is a powerful method for studying gene expression, but commonly used retroviral reverse transcriptases (e.g., MMLV and AMV) lack two critical properties for effective reverse transcription: intrinsic helicase activity, which enables the unwinding of complex RNA secondary structures, and high processivity, which allows for the synthesis of full-length cDNA from long RNA molecules.
UltraMarathonRT (uMRT) is a reverse transcriptase enzyme designed to overcome these limitations and improve RNA coverage. The cDNA Synthesis & Amplification Kit, powered by uMRT, combines intrinsic helicase activity with ultraprocessivity to efficiently reverse transcribe long RNA sequences (>30 kb—compared to the ~2 kb typically transcribed by retroviral RTs), as well as RNA with high GC content, complex structures, or low abundance. uMRT also functions at lower temperatures, helping preserve RNA integrity and enabling a more comprehensive view of the transcriptome.
Power through RNA complexity and boost sample coverage
The UltraMarathonRT cDNA Synthesis & Amplification Kit enables superior quantification of:
- Highly structured RNAs
- Long RNAs
- Transcripts with high GC content
- Non-coding RNAs
Ready to unveil more of the transcriptome?
Speak with our experts to learn how to get greater coverage without compromising on costs.