Get Longer Reads from your Direct RNA-Seq with UltraMarathonRT
This kit delivers >10% higher mapped reads from ≥ 2 kb RNAs. uMRT works at 30°C, which preserves the integrity of longer transcripts.
Optimized for Direct RNA-Seq
UltraMarathonRT delivers longer reads, higher yield, and superior isoform detection in Nanopore Direct RNA-Seq workflow.
As an exceptionally sensitive, helicase-driven enzyme, uMRT maintains continuous synthesis through structures, repetitive sequences, and modifications.
- 25-50% higher cDNA yield
- 5-10% longer read lengths, with very large differences in transcripts >2kb
- 1-2% more genes detected
- 15-20% more novel transcripts (NIC & NNIC) detected
- 25-30% more intron retention events detected
- Equivalent mapping rates (90-95%) & Q scores (17-18)
Become more confident in your detection of long transcripts, multiple isoforms, and modification analysis.
Number of mapped read counts per million (CPM) for read lengths ≥ 2 kb. Direct RNA sequencing performed on Human Brain Total RNA (Thermo, Cat#AM7962) using SQK-RNA004, N=2. All trials were down-sampled to 2.7M reads using SEQTK, then analyzed using NanoPlot.
Discover More Isoforms in your Direct RNA-Seq Data
UltraMarathonRT detects more isoforms per gene. Its intrinsic helicase activity unwinds RNA structures and enables full-length cDNA from each transcript.
Direct RNA Sequencing of Universal Human Reference RNA using uMRT and Induro. After down-sampling to an equal number of total reads, the reads were aligned using minimap2, and transcriptome models were generated using IsoQuant followed by curation using SQUANTI3. The SQUANTI3 analysis confirms that uMRT identifies more isoforms per gene compared to Induro.
Generate Higher cDNA Yields from your Sample
Capture more of the sample. UltraMarathonRT’s optimized chemistry delivers higher cDNA yields for Direct RNA-Seq.
The uMRT Direct RNA-Seq kit generates higher library yields. Direct RNA Sequencing libraries of Universal Human Reference RNA and Human Brain RNA were created using uMRT and Induro. Results show that uMRT generates ~ 25% more RNA/cDNA yield from 1 µg of total RNA.
Convenience & Cost Savings
The uMRT Direct RNA-Seq Kit contains everything you need to pair with ONT’s SQK-RNA004 – eliminating the cost, time, and hassle of ordering multiple different reagents from various vendors.
How uMRT Works - An Enzyme Engineered for Superior Processivity at Room Temperature
- Optimized Reaction Conditions: uMRT preserves RNA integrity by utilizing a 30°C reaction temperature
- Enhanced Processivity: Fully transcribes long RNA transcripts up to 30 kb in a single pass.
- Helicase-Driven Activity: Unwinds complex RNA structures to enable complete 5’ end to 3’ end sequencing of previously invisible regions.
Featured Resources
Beta Tester Feedback
“Long-read sequencing technologies have the amazing ability to access the full span of transcripts that can be kilobases or more in length. Unfortunately, most reverse transcriptases don’t. This was really hampering our ability to see the full spectrum of alternative splicing happening in transcripts, so discovering UltraMarathonRT has been a game-changer. The new uMRT DRS Kit seamlessly integrates into the ONT direct RNA sequencing protocol and gives us fantastic results. We will definitely use it for our direct RNA sequencing moving forward."
Dr. Paul Boutz, Assistant Professor, University of Rochester School of Medicine and Dentistry
Featured Publication
- Maio G, Guo L-T, Olson S, et al. Improved long transcript representation in Oxford Nanopore direct RNA sequencing with UltraMarathonRT. bioRxiv. 2025 Dec 10;2025.12.10.693495v1.
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