RT-PCR

A High-Performance Solution for Sensitive and Precise RNA Analysis

The UltraMarathonRT® Two-Step RT-PCR Kit is a next-generation tool designed for researchers seeking unmatched sensitivity, high-fidelity, and efficiency when analyzing RNA. Whether you are studying complex, long, or structured RNA targets, these kits deliver high-fidelity results with exceptional performance.

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Convert and Amplify with Precision

The UltraMarathonRT® Two-Step RT-PCR Kit offers faithful RNA-to-cDNA conversion followed by robust amplification, regardless of the RNA template’s length, secondary structure, or GC content.

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Key Features

Unparalleled
Sensitivity

Requires as little as 0.1 pg of input RNA to amplify RNAs >10 kb, enabling detection of even
low-abundance targets.

Sensitivity of uMRT two-step RT-PCR for RNA input as low as 0.1 pg

Powered by UltraMarathonRT®, an ultra-processive reverse transcriptase with intrinsic helicase activity that actively unwinds RNA secondary and tertiary structures without heat, producing full-length cDNA with exceptional fidelity.

Processive
Enzyme

Long-Read
Capability:

Efficiently amplifies cDNA products up to 30 kb using a highly processive, high-fidelity DNA polymerase.

Amplification of transcripts from 1.2 kb to 14.9 kb using 100 ng Hela total RNA. The cDNA’s generated by Induro RT were amplified by LongAmp Taq. Reverse transcription was performed using Oligo(dT) 18 primer.

Ideal for experiments requiring accurate gene detection, from short transcripts to tens of thousands of nucleotides in the areas of gene expression analysis, full-length isoform analysis, full-length virus genome analysis, molecular cloning, long or structured RNA analysis with low input.

Versatile
Applications

UltraMarathonRT Two-Step RT-PCR Kit

Perfect for researchers working with long, complex RNA templates who demand high-quality RT-PCR results with maximum sensitivity and precision.

Ready to try UltraMarathonRT for
RT-PCR?

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