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UltraMarathonRTTM Reverse Transcriptase Kit is designed to meet the evolving needs of the RNA research community. It is an ultra-processive reverse transcriptase that uses a single pass to create cDNAs from any type of RNA, including long, highly structured templates. Importantly, uMRT works optimally at ambient temperatures (20-42ºC) removing any need to heat the RT reaction which preserves the integrity of your RNA samples.
Used for gene expression, RT-PCR, qRT-PCR, and other first strand cDNA synthesis applications with RNA input as low as 0.1 pg.
UltraMarathonRT Reverse Transcriptase Kit components:
- UltraMarathonRT
- 2x RT Reaction Buffer
- High Boost
- Nuclease-free water
Catalog# R1002S (20 reactions), R1002M (50 reactions), R1002L (100 reactions)
For purchase orders please contact us at sales@rnaconnect.com.
- dNTP mix stock (10 mM each)
- The dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. High purity nucleotides (>99%) are recommended, and the pH should be adjusted to 7.0 with sodium hydroxide.
- Oligo-dT primer, random primer or gene specific primer
- RNA template: 1 pg-2 μg total cellular RNA or 1 pg-500 ng of mRNA are recommended for each reaction.
Storage conditions: For short duration of storage (< 3 month), UltraMarathonRT can be placed in a -20℃ freezer. -80℃ is recommended for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity.
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