2X qPCR Green Master Mix

RNAConnect's 2X qPCR Green Master Mix is a dye-based qPCR reagent optimized for accurate, reproducible real-time RNA quantification. Paired with full-length cDNA from a highly processive reverse transcriptase, such as UltraMarathonRT®, it delivers consistent C_t values regardless of qPCR primer pair location, removing a source of variability that affects RT-qPCR workflows built on non-processive retroviral RTs.

Key Features and Advantages

• Dye-Based Detection: Reliable dye-based chemistry for accurate, reproducible real-time monitoring of RNA levels on standard qPCR instruments.
  
• Consistent Quantification: When paired with full-length cDNA, provides consistent C_t values regardless of primer type (gene-specific, oligo(dT), or randomer) or amplicon location, as opposed to other master mixes, which can show widely different C_t values depending on where primers fall relative to GC-rich or structured regions.
• Fast Reaction Time: Run a full qPCR reaction in as little as ~35 minutes.
• Broad Compatibility: Works with cDNA from any first-strand synthesis reaction, though performance benefits (primer-position independence) are maximized when paired with UltraMarathonRT-generated cDNA.
  

Key Applications

• Gene expression quantification for RNA-Seq verification, alternative splice variant analysis, and mutation detection/allele discrimination
• Downstream quantification step for CRISPR knockdown/knockout confirmation, RNAi and ASO knockdown verification, RNA degradation, and RNA editing studies, when paired with UltraMarathonRT-generated cDNA
  

Kit Components

• 2x qPCR Green Dye Master Mix

Catalog#: Q1009S (200 rxns), Q1009M (500 rxns), Q1009L (1,000 rxns)

For the product brochure, click here; for purchase orders, contact us at sales@rnaconnect.com.

Reagents Required But Not Supplied

• cDNA template, such as first-strand cDNA generated with RNAConnect's UltraMarathonRT First-Strand cDNA Synthesis Kit (Cat. R1009).

Storage Conditions
The 2X qPCR Green Dye Master Mix is stable in a -20°C freezer for long-term storage and can endure 10 freeze-thaw cycles without losing noticeable activity.