{"product_id":"qpcr-green-dye-master-mix","title":"2X qPCR Green Master Mix","description":"\u003cp\u003e\u003cstrong\u003eRNAConnect's 2X qPCR Green Master Mix\u003c\/strong\u003e is a dye-based qPCR reagent optimized for accurate, reproducible real-time RNA quantification. Paired with full-length cDNA from a highly processive reverse transcriptase, such as UltraMarathonRT®, it delivers consistent \u003cspan style=\"mso-comment-continuation: 1;\"\u003eC\u003csub\u003et\u003c\/sub\u003e\u003c\/span\u003e values regardless of qPCR primer pair location, removing a source of variability that affects RT-qPCR workflows built on non-processive retroviral RTs.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKey Features and Advantages\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDye-Based Detection:\u003c\/strong\u003e Reliable dye-based chemistry for accurate, reproducible real-time monitoring of RNA levels on standard qPCR instruments.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConsistent Quantification: \u003c\/strong\u003eWhen paired with full-length cDNA, provides consistent \u003cspan style=\"mso-comment-continuation: 1;\"\u003eC\u003csub\u003et\u003c\/sub\u003e\u003c\/span\u003e values regardless of primer type (gene-specific, oligo(dT), or randomer) or amplicon location, as opposed to other master mixes, which can show widely different \u003cspan style=\"mso-comment-continuation: 1;\"\u003eC\u003csub\u003et \u003c\/sub\u003e\u003c\/span\u003evalues depending on where primers fall relative to GC-rich or structured regions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast Reaction Time:\u003c\/strong\u003e Run a full qPCR reaction in as little as ~35 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBroad Compatibility:\u003c\/strong\u003e Works with cDNA from any first-strand synthesis reaction, though performance benefits (primer-position independence) are maximized when paired with UltraMarathonRT-generated cDNA.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eKey Applications\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression quantification for RNA-Seq verification, alternative splice variant analysis, and mutation detection\/allele discrimination\u003c\/li\u003e\n\u003cli\u003eDownstream quantification step for CRISPR knockdown\/knockout confirmation, RNAi and ASO knockdown verification, RNA degradation, and RNA editing studies, when paired with UltraMarathonRT-generated cDNA\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eKit Components\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e2x qPCR Green Dye Master Mix\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eCatalog#: \u003c\/strong\u003eQ1009S (200 rxns), Q1009M (500 rxns), Q1009L (1,000 rxns)\u003c\/p\u003e\n\u003cp\u003eFor the product brochure, click \u003cspan style=\"color: rgb(43, 0, 255);\"\u003ehere\u003c\/span\u003e; for purchase orders, contact us at sales@rnaconnect.com.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReagents Required But Not Supplied\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ecDNA template, such as first-strand cDNA generated with RNAConnect's \u003ca href=\"https:\/\/rnaconnect.com\/products\/rt-qpcr-variants\" target=\"_blank\" rel=\"noopener\"\u003e\u003cspan style=\"color: rgb(43, 0, 255);\"\u003eUltraMarathonRT First-Strand cDNA Synthesis Kit (Cat. R1009)\u003c\/span\u003e\u003c\/a\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003cbr\u003eThe 2X qPCR Green Dye Master Mix is stable in a -20°C freezer for long-term storage and can endure 10 freeze-thaw cycles without losing noticeable activity. \u003cbr\u003e\u003c\/p\u003e","brand":"RNA-Connect","offers":[{"title":"150","offer_id":52743816642842,"sku":null,"price":120.0,"currency_code":"USD","in_stock":true},{"title":"500","offer_id":52743816675610,"sku":null,"price":360.0,"currency_code":"USD","in_stock":true},{"title":"1000","offer_id":52743816708378,"sku":null,"price":685.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0872\/5613\/8010\/files\/RNAConnect_2X_qPCR_Green_Master_Mix_Q1009M_1920x1080_cdcaa0df-c921-4a07-97b4-11519ed75ae0.jpg?v=1790703116","url":"https:\/\/rnaconnect.com\/products\/qpcr-green-dye-master-mix","provider":"RNAConnect","version":"1.0","type":"link"}